Dr. Rashmi K Shrestha

Assistant Professor of Biochemistry

Contact Information

Margaret A. Cargill Natural Sciences and Health Building, 418
Email: shresthar@berea.edu

Fall 2020

Office Hours

To be determined

Class Schedule

  • CHM 440
  • CHM 131B
  • CHM 370


  • B.S., University of Louisville, 2011
  • Ph.D., Purdue University, 2016 – Dissertation: Structural and Biochemical Characterization of Deubiquitinating Enzymes, Sst2 and Ssel
Rashmi Shrestha picture

About Me

I received a B.S. in Chemistry from University of Louisville in 2011. I then attended graduate school at Purdue University and earned my Ph.D. in Chemistry. During my graduate study, I structurally and biochemically characterized a yeast ubiquitin protease involved in lysosomal degradation of cell-surface receptors mediated by the macromolecular ESCRT machinery complexes. After graduate school, I worked as a molecular biologist in a biotech company focused on discovering and enhancing DNA promoters that regulate gene expression.  At the same time, I also taught Chemistry as an adjunct faculty in a community college in Oakland, CA. At UNCA, I am excited about teaching and mentoring students and working with Dr. Ryan Steed.

Awards and Affiliations

  • Purdue Summer Graduate Award, Purdue University, June 2015
  • American Society for Biochemistry and Molecular Biology (ASBMB) Graduate/ Postdoctoral Travel award, ASBMB Conference, March 2015
  • Women in Science Program (WISP) Travel Grant, Purdue University, November 2014
  • Intramural Research Incentive Grant, University of Louisville, December 2010
  • American Society for Biochemistry and Molecular Biology, November 2014 – Present
  • Phi Lambda Upsilon, Honorary Chemical Society, August 2012 – December 2016
  • Women in Science Program, September 2011 – December 2016

Papers and Publications

  • Shrestha, Rashmi, K.*, Bueno, Amy, N.* et al. Dynamics of an active-site flap contributes to catalysis in a JAMM family metallo deubiquitinase. 2015. Biochemistry. 54 (39); 6038–6051.
  • Shrestha, Rashmi, K.*, Ronau, Judith, A.* et al. Insights into the mechanism of deubiquitination by JAMM deubiquitinases from co-crystal structures of enzyme with substrate and product. 2014. Biochemistry. 53(19): 3199- 3217.
  • Newman, Justin, A. et al. Intercalating dyes for enhanced contrast in second-harmonic generation imaging of protein crystals. 2015. Acta Crystallogr D Biol Crystallogr. 71 (Pt 7):1471-7.
  • Shrestha, Rashmi, K.et al. Analysis of the Composition of Lipid in Human Meibum from Normal Infants, Children, Adolescents, Adults and Adults with Meibomian Gland Dysfunction using 1H-NMR Spectroscopy. 2011. Invest Ophthalmol Vis Sci. 52:7350-7358.

Poster Presentations

  • The 35th Midwest Enzyme Chemistry Conference, October 2015: “Dynamics of an Active-site Flap in JAMM Deubiquitinases Contributes to Catalysis.”
  • Purdue Teaching Academy Day Poster Session, September 2015: “Evaluation of Energy and Thermodynamics Content in a General Chemistry Course.”
  • Chemical-Biology Interface Career Development Poster Session, August 2015: “Dynamics of an Active-site Flap in JAMM Deubiquitinases Contributes to Catalysis.”
  • American Society for Biochemistry and Molecular Biology Conference, March 2015: “Understanding the Molecular Basis of MIC-CAP Syndrome through Structural and Functional Studies of the Deubiquitinase AMSH.”.
  • The 34th Midwest Enzyme Chemistry Conference, September 2014: “Understanding the Mechanism of Endosomal Deubiquitinase AMSH.”
  • 31st Annual H.C. Brown Symposium, April 2014: “Endosomal Deubiquitinase AMSH is regulated by Ubiquitin binding.”
  • Sigma Xi Graduate Student Research Poster Session, February 2014: “Endosomal Deubiquitinase AMSH is regulated by Ubiquitin binding.”
  • Ubiquitination Processes and Their Role in Cancer, April 2013: “Structures of the Endosomal Deubiquitinase Sst2 from S. pombe and its Product Complex.”